Methods of the experimental work

Course aim (aims)

1. Getting to know the principles of the safe work in the laboratory.
2. Getting to know the possibilities of scientific career.
3. Getting to know the basic tools and devices used in the laboratory.
4. Transfer of knowledge concerning the genome, transcriptome and proteome.
5. Transfer of knowledge concerning the principles of the operation and using modern research tools such as: Real Time PCR technique, Droplet Digital PCR (ddPCR), deep sequencing (NGS, Illumina, Nanopore), FLIM-FRET, CRISPR/Cas9.
6. Transfer of knowledge regarding the use of bioinformatics tools for analyzing NGS data (CLC Genomics Workbench), for analyzing protein structures (NovaFold). Acquiring skills in Linux commands usage (Ubuntu).
7. To develop skills to correctly use basic statistical operations: mean, median, standard deviation, p-value, corrected p-value, Poisson statistic.
8. Developing the ability to plan and conduct correctly experiments and analyze the obtained results. The research topic presented in the academic year 2020/2021 are - diseases caused by triplet nucleotide repeats - Triplet Repeat Expansion Diseases (TREDs).
9. Developing skills related to the preparation of student’s own research project and group discussions on the strengths and weaknesses of the particular project.