Digital PCR

Course aim (aims)

1. Transfer of knowledge concerning operation and use Real-time PCR (qPCR) technology.
2. Transfer of knowledge concerning droplet digital PCR (ddPCR) and differences between conventional qPCR and ddPCR.
3. Develop skills associated with cDNA preparation, ddPCR reaction set up.
4. Transfer of knowledge concerning the use of ddPCR technique in gene expression analysis, Single Nucleotide Polymorphism detection (SNP), in viral RNA detection, detection of genome editing events generated by CRISPR/Cas9 technique used in biotechnology.
5. Transfer the knowledge of proper use of basic statistics and ddPCR calculations necessary for ddPCR data analysis: mean, median, standard deviation, T-test, Poisson statistic.
6. Develop skills associated with the preparation of student’s own results evaluation report.